Analysis Note

ControlRat basophil leukemia (RBL) cell lysate

Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Flow Cytometry Analysis: 0.5 µg from a representative lot detected NLRP12 in rat basophil leukemia (RBL).

Anti-NLRP12 Antibody is a highly specific rabbit polyclonal antibody, that targets NLRP & has been tested in western blotting & Flow Cytometry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NLRP12 (NACHT, LRR and PYD domains-containing protein 12) is also known as Monarch-1 or PYRIN-containing APAF1-like protein 7 (PYPAF7). NLRP12 is thought to mediate activation of CASP1 (via ASC) and NF-kappa-B (via IKK). NLRP12 has been detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes, and is regulated by nitric oxide. NLRP12 defects can cause familial cold autoinflammatory syndrome type 2 (FCAS2).

Immunogen

KLH-conjugated linear peptide corresponding to human NLRP12.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in rat basophil leukemia (RBL) cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NLRP12 in 10 µg of rat basophil leukemia (RBL) cell lysate.

Specificity

Other homologies: Mouse (91% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~110 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing this antibody recognizes isoforms 1 through 4 at ~120 kDa, ~114 kDa, ~107 kDa, and ~102 kDa