Application

Research CategorySignaling

Anti-Mff, Cat. No. ABS2087, is a rabbit polyclonal antibody that detects Mitochondrial fission factor (Mff) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Western Blotting Analysis: A representative lot detected Mff in Western Blotting applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44).

Immunofluorescence Analysis: A representative lot detected Mff in Immunofluorescence applications (Mishra, P., et. al. (2015). Cell Metab. 22(6):1033-44).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mitochondrial fission factor (UniProt: Q9GZY8; also known as Mff) is encoded by the MFF (also known as C2orf33, AD030, AD033, GL004) gene (Gene ID: 56947) in human. Mff is a single-pass type IV membrane protein that is highly expressed in heart, kidney, liver, brain, muscle, and stomach. Mff is a homodimeric protein, but may also interact with other proteins. It plays a role in mitochondrial and peroxisomal fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. It is also suggested to be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. Mff has a cytoplasmic domain (aa 1-322), a helical domain (aa 323-340), and a mitochondrial intermembrane domain (aa 341-342). Mff is shown to be an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells and its down-regulation inhibits mitochondrial recruitment of Drp1 and induces mitochondria elongation. Also, exogenous expression of Mff is shown to induce extensive mitochondrial fission. Mutations in MFF gene can cause a form of encephalopathy that is characterized by delayed psychomotor development, severe hypotonia with inability to walk, and microcephaly. (Ref.: Otera, H., et al. (2010). J. Cell Biol. 191 (6); 1141 1158; Gandre-Babbe, S., and van der Bliek, AM. (2008). Mol. Biol. Cell. 19(6): 2402-2412).

Immunogen

His-taged full length human recombinant mitochondrial fission factor (Mff).

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.

Format: Unpurified

Unpurified

Quality

Evaluated by Immunocytochemistry in HeLa cells with Mitotracker (TM).

Immunocytochemistry Analysis: A 1:2,000 dilution of this antibody detected Mff in HeLa cells with Mitotracker (TM).

Specificity

This rabbit polyclonal antibody detects mitochondrial fission factor (Mff) in human cells.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

38.47 kDa calculated.