Application

Research CategoryNeuroscience

Anti-MARCO, Cat. No. ABN1389, is a highly specific rabbit polyclonal antibody that targets Macrophage receptor MARCO and has been tested in Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected MARCO in 10 µg of A549 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Macrophage receptor MARCO (UniProt: Q9UEW3; also known as macrophage receptor with collagenous structure; Scavenger receptor class A member 2) is encoded by the MARCO (also known as SCARA2) gene (Gene ID: 8685) in human. MARCO is strongly expressed in macrophages of several tissues in humans with sepsis. It is a scavenger receptor that is readily upregulated following bacterial infection. Hence, it forms a back-up system through which the organism can quickly arm more cells with highly efficient phagocytosing receptors. MARCO is a single-pass type II membrane protein that binds both Gram-positive and Gram-negative bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. The bacteria-binding region is localized immediately proximal to the cysteine-rich region of domain V. Human and mouse MARCO exhibit about 68% sequence identity. However, the human MARCO subunit chain differs from the mouse chain in that it does not have any cysteine residues outside the cysteine-rich domain V, whereas the mouse chain has one cysteine residue within the intracellular domain I and two in the extracellular spacer domain III. (Ref.: Elomaa, O., et al. (1998). J. Biol. Chem. 273(8): 4350-4538).

Immunogen

KLH-cojugated peptide corresponding to 12 amino acids from the C-terminal region of human MARCO.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Affinity Purified

Quality

Evaluated by Western Blotting in RAW264.7 cells.

Western Blotting Analysis (WB): A 1:5000 Dilution of this antibody detected Macrophage receptor MARCO in 10 µg RAW264.7 cells .

Specificity

This polyclonal antibody detects macrophage receptor MARCO in RAW264.7 cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed; 52.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).