Analysis Note

ControlHuman lymph node tissue lysate

Application

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LIPE in human heart and lipoma tissues.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected LIPE in human lymph node cells.

Detect Hormone Sensitive Lipase using this rabbit polyclonal antibody, Anti-LIPE Antibody validated for use in western blotting, IHC & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Hormone-specific lipase (HSL, LIPE) is a member of the GDXG lipolytic enzyme family. LIPE is found in the high-density caveolae and upon insulin stimulation it translocates to the cytoplasm. LIPE is expressed in multiple tissues and cell lines and it plays important role in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. LIPE has been shown to hydrolyze stored triglycerides to free fatty acids in adipose tissue and heart; while in steroidogenic tissues, it converts cholesteryl esters to free cholesterol for steroid hormone production. Recent studies suggest that the lack of LIPE in genetically obese leptin-null mice inhibit obesity and adipogenesis, and further implying that LIPE plays a major role in adipocyte proliferation.

Immunogen

KLH-conjugated linear peptide corresponding to human LIPE.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in human lymph node tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LIPE in 15 µg of human lymph node tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~88 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~84 kDa and ~117 kDa