Analysis Note

ControlHuman brain tissue lysate

Application

Research CategoryNeuroscience

Detect the LGI4 protein using this Anti-LGI4 validated for use in WB.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs). Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with autodominant lateral temporal epilepsy (ADTLE). However, the LGI4 gene is located in a region linked to benign familial infantile convulsions and a GC-to-AT polymorphism in LGI4 was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.

Immunogen

KLH-conjugated linear peptide corresponding to human LGI4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in human brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected LGI4 in 15 µg of human brain tissue lysate.

Specificity

Two isoforms of LGI4 are known to exist; this LGI4 antibody will recognize only the larger form. This LGI4 antibody is predicted to be specific to LGI4 and not recognize other LGI proteins.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~59 kDa observed