Analysis Note

ControlHuman kidney tissue lysate

Application

Immunohistochemistry Analysis: A 1:200 dilution of Anti-LCN2 antibody from a previous lot detected LCN2 in human spleen tissue.

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Anti-LCN2 Antibody detects level of LCN2 & has been published & validated for use in WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

LCN2 (also known as Neurophil Gelatinase-Associated Lipocalin or NGAL) belongs to a superfamily of lipocalins, a collection of small, diversely functional, extracellular proteins. NGAL is secreted from neutrophil granules in three different forms; a monomer of 25 kDa, a disulfide-linked homodimer at 46 kDa, and a disulfide-linked heterodimer with MMP-9 at 135-kDa. It is widely known to induce development of kidney epithelia, to bind and traffic iron, and to act as a molecular signal under inflammatory conditions, such as Crohn's Disease. Multiple carcinoma studies have also shown elevated expression levels of LCN2 in several different human tumors affecting such organs as: colon, lung, breast, ovaries and pancreas. Additionally, there has been great interest in this protein as a possible marker for: onset of Acute Kidney Injury following cardiac surgery, the progression of Chronic Kidney Disease (CKD), and the survival chances of patients with Chronic Heart Failure (CHF).

Immunogen

Epitope: Mature chain

KLH-conjugated linear peptide corresponding to the mature chain of human LCN2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected LCN2 in 10 µg of human kidney tissue lysate.

Specificity

This antibody recognizes LCN2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

LCN2 band observed at 25 kDa Heavy concentration of tissue lysate and a 5 minute exposure time were used to generate the band of interest.