Application

Research CategorySignaling

Western Blotting Analysis: A representative lot detected glutathione S-transferase P in rat liver and human stomach tissue homogenates (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Western Blotting Analysis: A representative lot detected upregulated glutathione S-transferase P (GSTP1) levels in liver homogenates from rats on diets supplemented with either one of six (EQ, CMRN, BHA, DEM, t-SO, or β-NF), but not with four other phytochemicals (OPZ, I3C, BITC, or PB) tested (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Immunohistochemistry Analysis: A representative lot detected much reduced glutathione S-transferase P (GSTP1) immunoreactivity in paraffin-embedded Barrett′s oesophagus tissues when compared to normal human oesophagus tissue sections (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Immunohistochemistry Analysis: A representative lot detected enhanced Glutathione S-transferase P immunoreactivity in the centrilobular part of liver from rats received dietary coumarin (CMRN) using acetone-fixed, paraffin-embedded tissue sections (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).

Anti-Gstp1 Antibody is an antibody against Gstp1 for use in Western Blotting, Immunohistochemistry.

Research Sub CategoryOxidative Stress

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutathione S-transferase P (EC 2.5.1.18; UniProt P04906; also known as Chain 7, GST 7-7, GST class-pi) is encoded by the Gstp1 gene (Gene ID 24426) in rat species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTP exists as a homodimer composed of two Yf subunits.

Immunogen

Purified rat liver glutathione S-transferase P.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTP1 Yf monomer in 10 µg of human lung tissue lysate.

Specificity

Reacts with human and rat glutathione S-transferase P dimer (Yf/Yf) and monomer (Yf).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~25 kDa observed (Yf monomer).