Analysis Note

ControlHeLa cell lysates.

Application

Research CategorySignaling

Research Sub CategoryRNA Binding Protein (RBP)

Anti-G3BP1 Antibody detects level of G3BP1 & has been published & validated for use in WB & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). It was first discovered by its binding to RasGAP. G3BPs have been shown to be involved in a number of mitogenic signaling pathways. G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007). Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs.

Immunogen

Epitope: C-terminus

Synthetic peptide corresponding to C-terminal region of human G3BP1.

Physical form

Rabbit polyclonal serum with 0.05% NaN3.

Quality

Evaluated by western blot on HeLa cell lysates.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect G3BP1 in HeLa, 293T and S10 cell lysates.

Specificity

This antibody detects G3BP1 at its C-terminal region.

Not tested on other species.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

68 kDa