Analysis Note

ControlHuman heart lysate.

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

This Anti-Frataxin Antibody is validated for use in WB for the detection of Frataxin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Frataxin is a nuclear encoded protein that is found in the mitochondria. This protein is not well understood but may be involved with iron sulfur clusters. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. The specific function of Frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have demonstrated that the deletion of the Frataxin gene in yeast results in iron accumulation in mitochondria and loss of respiration. Recombinant human Frataxin has been shown to bind iron in vitro, and increased mitochondrial iron levels have been observed in patients with Friedreich′s Ataxia (FRDA).

Immunogen

Epitope: mature chain

GST-tagged Frataxin corresponding to mature chain.

Physical form

GST-depleted sera

Rabbit polyclonal serum in buffer containing 0.05% NaN3.

Format: Purified

Quality

Routinely evaluated by Western Blot on Human heart lysates.

Western Blot Analysis: 1:1,000 - 1:10,000 dilution of this lot detected Frataxin on 10 ug of human heart lysate

Specificity

Cat. # AB15080 recognizes Frataxin.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

Approx. 18 kDa