Application

Research CategoryInflammation & Immunology

Flow Cytometry Analysis: 1.0 µg of this antibody detected FPR2 in HL-60 cells.

Anti-FPR2 Antibody | ABF118 is an antibody against FPR2 Antibody for use in Western Blotting, Flow Cytometry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Formyl peptide receptors (FPRs) are G protein coupled receptors critical for our immune response and the cellular activation and or suppression of immune cell activities. N-formyl peptides are produced by the degradation of either bacteria or host cells and serve as chemoattractant agents for white blood cells. There are three known formyl peptide receptors, FPR1, FPR2, and FPR3 all appear to have similar functions. Binding of N-formyl peptides activates neutrophils via the phosphatidylinositol-calcium second messenger system. In some cases binding leads to a down regulation of immune signals such as in the down regulation observed against the proinflammatory signaling agent leukotriene B4. Interestingly, FPRs also have endogenous ligands which appear to function in a developmental fashion and not directly as an immune function. For instance, FPR2’s endogenous ligand is a peptide named Cathelin-related antimicrobial peptide (CRAMP) which is critical for proper dendritic cell maturation. FRP2 is abundantly expressed in the lung, spleen and testis neutrophils. Because of their role in the immune cell activation, FPRs are also being investigated as possible targets for modulating immune caused diseases such as arthritis.

Immunogen

Epitope: extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of human FPR2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FPR2 in 10 µg of HL-60 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed. The calculated molecular weight of this protein is 39 kDa, but can be observed at ~45 kDa due to high glycosylation.