Anti-FAF1

Analysis Note

ControlJurkat cell lysate

Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected FAF1 in rat spleen tissue.

Anti-FAF1 Antibody is a highly specific rabbit polyclonal antibody, that targets Fas-associated protein 1 & has been tested in western blotting & IHC.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fas-associated protein 1 (FAF1) is a member of the TNF receptor superfamily that plays a critical role in apoptosis during development and immune function. FAF1 has two amino-terminal domains with structural homology to ubiquitin. FAF1 has been observed to be one of the components of the death-inducing signaling complex (DISC) during Fas-mediated apoptosis and can bind to caspase-8 and FADD. Additionally, FAF1 has been shown to suppress the activation of the NF-kappaB transcription factor. FAF1 is also known to interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. FAF1 is widely expressed with highest expression observed in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human FAF1.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected FAF1 in 15 µg of Jurkat cell lysate.

Specificity

This antibody recognizes the C-terminus of FAF1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~78 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~57 kDa and ~74 kDa