Analysis Note

ControlA431 cell lysate

Application

Research Sub CategoryRNA Binding Protein (RBP)

Research CategoryEpigenetics & Nuclear Function

Anti-eIF4A3 Antibody is a rabbit polyclonal antibody for detection of eIF4A3 also known as ATP-dependent RNA helicase DDX48, Eukaryotic translation initiation factor 4A isoform 3 & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Eukaryotic translation initiation factor 4A, isoform 3, also known as eIF4A3, is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Immunogen

KLH-conjugated synthetic linear peptide corresponding to eIF4A3.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by western blot on A431 cell lysate.
Western Blot Analysis: 1:1000 dilution of this antibody detected eIF4A3 in A431 cell lysate.

Specificity

This antibody detects RNA-binding protein eIF4A3.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~34 kDa