Analysis Note

ControlK562 cell lysate

Application

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected EBI3 in human spleen tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected EBI3 in human spleen cells.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryCell Structure

Detect IL-27 using this rabbit polyclonal antibody, Anti-EBI3 Antibody validated for use in western blotting, IHC & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epstein-Barr virus-induced gene 3 protein (EBI3), also known as Interleukin-27 subunit beta (IL27B), is a member of the type I cytokine receptor family (Type 3 subfamily) that is composed of two subunits: interleukin-27 (IL-27) and IL-35. EBI3 regulates T-helper cell development, stimulates cytotoxic T-cell activity, suppresses T-cell proliferation, and induces isotype switching in B-cells. EBI3 also plays an important role in its antitumor activity as well as its antiangiogenic activity.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human EBI3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in K562 cell lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected EBI3 in 15 µg of K562 cell lysate.

Specificity

This antibody recognizes the C-terminus of EBI3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed. Uncharacterized bands may appear in some lysate(s).