Analysis Note

ControlRat Brain lysate.

Application

Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant Peptide.

Epitope: C-Terminus

Linkage

Replaces: AB5910

Physical form

GST-Depleted Sera with 0.05% NaN3 .

GST Depletion

Quality

Evaluated by Western Blotting in rat brain tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.

Specificity

This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.

Reactivity with other species has not been determined.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).