Analysis Note

ControlSK-N-SH cell lysate

Application

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected CHD7 in mouse brain tissue.

Anti-CHD7 Antibody is a highly specific rabbit polyclonal antibody, that targets CHD7 & has been tested in western blotting & IHC.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chromodomain-helicase-DNA-binding protein 7 (CHD7), also known as ATP-dependent helicase CHD7, belongs to a superfamily of proteins of the ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. CHD7 may play an important role in early embryonic development by affecting chromatin structure and gene expression. CHD7 interacts with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes. Defects in CHD7 are suggested to be the cause of CHARGE syndrome (CHARGES) and idiopathic scoliosis type 3 (IS3). Mutations in CHD7 may also result in hypogonadotropic hypogonadism (HH5). CHD7 is widely expressed in fetal and adult tissues.

Immunogen

KLH-conjugated linear peptide corresponding to human CHD7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in SK-N-SH cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CHD7 in 15 µg of SK-N-SH cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~290 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~128 kDa and ~336 kDa