Analysis Note

ControlHepG2 cell lysate

Application

Western Blot Analysis: 2.5 µg/mL from a representative lot detected CCDC53 in 10 µg of mouse brain and PC12 cell lysate.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

This Anti-CCDC53 Antibody is validated for use in WB for the detection of CCDC53.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Coiled-coil domain-containing protein 53 (CCDC53) is one of several components comprising the WASH complex, a complex localized to endosomal surfaces that functions to upregulate actin polymerization through the recruiting and activation of Arp2/3 complex. WASH is a critical component in the fission of tubules (transport intermediates involved in the distribution of endosomes). The WASH complex is composed of CCDC53, F-actin-capping protein subunits alpha and beta, WASH, FAM21, KIAA1033, and KIAA196.

Immunogen

GST-tagged recombinant protein corresponding to human CCDC53.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected CCDC53 in 10 µg of HepG2 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~28 kDa observed