Application

Research CategoryApoptosis & Cancer

Western Blotting Analysis: 2 µg/mL from a representative lot detected Bif-1 in 10 µg of Jurkat cells and human kidney lysates.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Bif-1 in human placenta, human skin, human small intestine, and human testis tissues.

Anti-Bif-1, Cat. No. ABC947, is a rabbit polyclonal antibody that detects Bax-interacting factor 1 (Bif-1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Endophilin-B1 (UniProt: Q9Y371; also known as Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1) is encoded by the SH3GLB1 (KIAA0491; CGI-61) gene (Gene ID: 51100) in human.
Bif-1 is a member of the endophilin protein family that forms a complex with Beclin-1 through ultraviolet irradiation resistant-associated gene (UVRAG) and promotes the activation of the class III PI3 kinase, Vps34, in mammalian cells. It is highly expressed in heart, skeletal muscle, kidney, and placenta and is detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung, and peripheral blood leukocytes. Loss of Bif-1 significantly suppresses PI3KC3 activation and the formation of autophagosome in both HeLa cells and mouse embryonic fibroblasts (MEFs). Bif-1 is reported to be involved in mitochondrial fission and coat protein complex I (COPI)-vesicle formation. Bif-1 contains a BAR domain at the N-terminal (aa 27-261) and a SH3 domain at the C-terminal (305-365), which is required for interaction with UVRAG. Bif-1 may be present as a homodimer or as a heterodimer with Endophilin-B2 (SH3GLB2). Bif-1 can be phosphorylated at Threonine 145 by Cdk5 and this phosphorylation is required for autophagy induction in starved neurons. Three isoforms of Bif-1 have been reported that are generated by alternative splicing. Isoform 1 acts as pro-apoptotic molecule in fibroblasts and is involved in caspase-independent apoptosis during nutrition starvation and in the regulation of autophagy. Isoform 2 has an anti-apoptotic role in neuronal cells and is involved in maintenance of mitochondrial morphology and promotes neuronal viability. (Ref.: Takahashi, Y et al (2009). Cell Death Differen. 16(7), 947-955).

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Bax interacting factor 1 (Bif-1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Bif-1 in 10 µg of HEK293 cell lysate.

Specificity

This rabbit polyclonal antibody detects Bax interacting facor 1 (Bif-1) in human cells. it targets an epitope within 15 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~52 kDa observed; 40.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).