Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Beclin-2 in human brain (thalamic region) and mouse stomach tissues.

Anti-Beclin-2 Antibody, Cat. No. ABC253, is a rabbit polyclonal antibdoy that detects Beclin-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Beclin-2 (UniProt: P0DM65) is encoded by Becn2 gene (Gene ID: 226720) in murine species. Beclin-2 is a member of the Atg6/Beclin family and is involved in 2 distinct lysosomal degradation pathways and acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. It regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. Beclin-2 family members are specific to mammals whereas Becln-1 subfamily members are present in more primitive vertebrates. Beclin-2 interacts with ATG14, AMBRA1, UVRAG and PIK3C3/VPS34 and this interaction is not disrupted by starvation. It is expressed in brain, skeletal muscle, placenta, thymus and uterus and expression is lower in liver, testis, stomach, and 17-day-old embryos. Disruption of Beclin-2 is shown to reduce embryonic viability and heterozygous knockout mice show defects in autophagy, increased levels of Cnr1 receptor, elevated food intake, and obesity and insulin resistance. (Ref.: He, C. et al. (2013). Cell 154, 1085-1099).

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Beclin-2.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in Beclin2 +/+ Mouse Brain (Wild-Type).

Western Blotting Analysis: 2 µg/mL of this antibody detected Beclin-2 in lysate from Beclin-2 +/+ Mouse Brain (Wild-type).

Specificity

This rabbit polyclonal antibody detects Becline-2 in murine cells. It targets an epitope within 14 amino acids from the N-terminal.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed; 50.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).