Analysis Note

ControlHL-60 cell lysate

Application

Anti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATG3 is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE). This compound is necessary for autophagy, which is a process in which a cell is degraded by its own lysosomal process.

Immunogen

GST-tagged recombinant protein corresponding to human ATG3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ATG3 in 10 µg of HL-60 cell lysate.

Specificity

Other homologies: Ape, rhesus macaque, equine, porcine, and bovine (99% sequence homology). Mouse (98% sequence homology). Rat (97% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed