Anti-ASF1a

Code: ABE149 D2-231

Analysis Note

ControlHeLa cell lysate

Application

Research CategoryEpigenetics & Nuclear Function

Anti-ASF1a Antibody is a Rabbit Polyclona...


read more

Your Price
€500.00 EACH
€615.00 inc. VAT

Analysis Note

ControlHeLa cell lysate

Application

Research CategoryEpigenetics & Nuclear Function

Anti-ASF1a Antibody is a Rabbit Polyclonal Antibody for detection of ASF1a also known as Histone chaperone ASF1A or hAsf1 & has been validated in WB & IP.

Research Sub CategoryChromatin Biology

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Banumathy, G, et al. (2009). Mol Cell Biol. 29(3):758–770.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. This protein facilitates histone placement and histone removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). ASF1a interacts with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA inside PML bodies of cells approaching senescence after p53/p21 activation.

Immunogen

GST-tagged recombinant protein corresponding to human ASF1a.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected ASF1a on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~23 kDa observed

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... ASF1A(25842)
NCBI accession no.NP_054753
purified byaffinity chromatography
Quality Level100
shipped inwet ice
species reactivityhuman, rat, mouse
technique(s)western blot: suitable, immunoprecipitation (IP): suitable
UniProt accession no.Q9Y294
This product has met the following criteria to qualify for the following awards:



PROCEED TO CHECKOUT

HAVE AN ACCOUNT? LOGIN


GUEST CHECKOUT

Proceed as a guest. You will have the option to register to access exclusive pricing and stock availability features after checkout.