Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARL13B in human cerebellum, embryonic mouse liver, and human cerebral cortex tissue.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Anti-ARL13B Antibody is an antibody against ARL13B for use in Immunohistochemistry and Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ARL13B/ARL2L1 is an important neuronal developmental protein, as well as, a cilium-specific protein required for the control of the microtubule-based ciliary axoneme structure. ARL13B/ARL2L1 is required for proper patterning in neural development during neural tube formation, and ARL13B/ARL2L1 is critical for cerebral cortex development and the initial polarization of radial glia scaffold, the first step in cortex development. ARL13B/ARL2L1 also controls the migration and placement of post mitotic interneurons. ARL13B/ARL2L1 is found in cellular projections and cilium and is widely expressed in the developing system as well in the gut. ARL13B/ARL2L1 also is a member of the small GTPase superfamily as it binds GTP but does not cleave it.

Immunogen

KLH-conjugated linear peptide corresponding to human ARL13B.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse E13-14 brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ARL13B in 10 µg of mouse E13-14 brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed. Western blot shows the absence of a 60 kDa Arl13b band in Arl13bE9.5 (Humbert, M.C., et al. (2012). PNAS. 109(48):19691-19696).