Analysis Note

ControlA431 cell lysate

Application

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adam17 in 10 µg of the following cell lysates--C2C12, HEK293, HeLa, HepG2, HUVEC, L6, NIH/3T3, PC12, and PC3.

Western Blotting Analysis: A representative lot detected pro-form and the truncated, mature form of Adam17 in COS cell lysates (Schlondorff, J., et al. (2000) Biochem J. 1(347):131-138.).

Western Blotting Analysis: A representative lot detected Adam17 in COS-7 cell lysates (Zheng, Y., et al. (2002). J Biol Chem. 277(45):42463-42470.).

Western Blotting Analysis: A representative lot detected the Pro-form and the truncated. mature form of Adam17 in COS-7 cell lysates (Le Gall, S. M., et al. (2010). J Cell Sci. 123(22):3913-3922.).

Research CategoryCell Structure

Anti-Adam17 Antibody is a highly specific rabbit polyclonal antibody, that targets ADAM 17 & has been tested in western blotting & ICC.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ADAM17, also known as TNF-alpha Convertase, or TACE, was first cloned from human epithelial cells. Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26 kDa Type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. The ADAM proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane, and a cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metallo-proteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.

Immunogen

GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Adam17.

Epitope: Cytoplasmic domain

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adam17 in 10 µg of A431 cell lysate.

Specificity

This antibody recognizes the cytoplasmic domain of Adam17.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~120 kDa and ~100 kDa observed. The pro-form and the truncated, mature form of Adam17 may be observed at 120 kDa and 100 kDa, respectively (Schlondorff, J., et al. (2000) Biochem J. 1(347):131-138.; Le Gall, S. M., et al. (2010). J Cell Sci. 123(22):3913-3922.).