Analysis Note

ControlFor 07-681SP use HEK293 cells or rat liver AMPK.For 07-363SP use Rat liver preparation.For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.

Application

Research CategorySignalingApoptosis & Cancer

Research Sub CategoryInsulin/Energy Signaling

This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).

Components

07-350SP Anti-AMPK α1; 60 µg07-363SP Anti-AMPKα2; 60 µg07-681SP Anti-phospho-AMPKα (Thr172); 60 µg

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).

AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

*See full size versions for corresponding references.

Immunogen

Epitope: 07-350SP and 07-363SP: Internal

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Each vial is 30% the size of the parent catalog number

Physical form

Protein A purified

3 individual tubes containing either Anti-AMPK α1; Anti-AMPKα2; or Anti-phospho-AMPKα (Thr172)

Format: Purified

Quality

07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.

07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.

Western Blot Analysis:
1-2 µg/mL of this lot detected AMPK α2 in a rat liver preparation.


07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.

Specificity

07-681SP: Expected to cross-react with mouse based on 100% sequence homology.

Cross ReactivtyVaries. See individual Data Sheets

07-681SP: AMPKα phosphorylated on Thr172.

07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).

07-350SP: AMPKα1

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Target description

~63 kDa;