Application

4-(2-(Hex-5-ynamido)ethyl)benzenesulfonyl fluoride is an alkyne-functionalized sulfonyl fluoride activity-based probe (SFABP). Sulfonyl fluorides covalently modify reactive serines in addition to threonine, lysine, tyrosine, cysteine, and histidine residues in a context-specific manner. Activity-based probes are useful for the targeting, isolation, and identification of proteins. Furtermore, the alkyne handle on SFABP enables click chemistry-based incorpoation of azide tags, such as fluorophores or biotins, for visualization or enrichment and detection, respectively.

Other Notes

Unbiased Mass Spectrometry Elucidation of the Targets and Mechanisms of Activity-Based Probes: A Case Study Involving Sulfonyl FluoridesSulfonyl fluorides as privileged warheads in chemical biologyChemical Proteomics with Sulfonyl Fluoride Probes Reveals Selective Labeling of Functional Tyrosines in Glutathione TransferaseSulfonyl Fluoride Analogues as Activity-Based Probes for Serine ProteasesEuropium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS