Trizma hydrochloride, BioUltra, for molecular biology, >=99.0% (AT)

Code: 93363-100G D2-231

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for...


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Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.

λ4 M in H2O
anion tracessulfate (SO42-): ≤50 mg/kg
assay≥99.0% (AT)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
formpowder or crystals
gradefor molecular biology
ign. residue≤0.2% (as SO4)
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI keyQKNYBSVHEMOAJP-UHFFFAOYSA-N
InChI1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
loss≤0.2% loss on drying, 110 °C
mp150-152 °C
pH2.5-4.0 (25 °C, 4 M in H2O)
pKa (25 °C)8.1
product lineBioUltra
Quality Level200
SMILES stringCl.NC(CO)(CO)CO
solubilityH2O: 4 M at 20 °C, clear, colorless
useful pH range7.0-9.0
UV absorptionλ: 280 nm Amax: 0.08, λ: 260 nm Amax: 0.12
Cas Number1185-53-1
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