Rhodamine 123, BioReagent, for fluorescence, >=85% (HPLC)

Application

Rhodamine 123 has been used:as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assaysto label the cells for estimating the P-gp expression by flow cytometryin transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

General description

Rhodamine 123 is a fluorescent dye.

Other Notes

Supravital mitochondrial-specific dye