Anti-GAD67, clone 1G10.2 ZooMAb�MsMono

Application

Anti-GAD67, clone 1G10.2 ZooMAb, Cat. No. ZMS5406, is a recombinant Mouse monoclonal antibody that specifically targets Glutamate decarboxylase 1 (GAD67) and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAD67 in mouse brain tissue lysate.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 cortical cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAD67 in mouse brain and rat brain tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

Recombinant human Glutamate decarboxylase 1 (GAD67).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1G10.2 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Glutamate decarboxylase 1 (GAD67).

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Glutamate decarboxylase 1 (UniProt: Q99259; also known as EC:4.1.1.15, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform) is encoded by the GAD1 (also known as GAD, GAD67) gene (Gene ID:2571) in human. Glutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD have been described (65 kDa and 67 kDa) that display 64% amino acid homology and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct, and ovary. Between the two isoforms, GAD67 is responsible for over 90% of basal GABA synthesis and is produced at limiting levels in the brain. These isoforms are regionally distributed in cytoplasm in the brains of rats and mice. GAD67 expression is shown to change during neural development in rat spinal cord and its expression seems to be particularly plastic and can change in response to experimental manipulation and disease progression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.