Application

Research CategoryCell Structure

Anti-Plakophilin-3, clone 7F6, Cat. No. MABT1496, is a mouse monoclonal antibody that detect Plakophilin-3 and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Plakophilin-3 (UniProt: Q9Y446; also known as PKP3) is encoded by the PKP3 gene (Gene ID: 11187) in human. Plakophilin 3 a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells and dendritic reticular cells of lymphatic follicles. Plakophilin-3 is an Armadillo-like protein whose expression pattern is largely restricted to epithelial cell types and plays a role in junctional plaques. It is localized in the desmosomal plaques and in the nucleus. Plakophilin-3 contains a central Arm repeat domain, containing 10 Arm repeats and a short C-terminal domain. Plakophilin proteins act in a cellular desmosome-dependent adhesion and signaling pathways. It is shown to bind directly to the desmosomal cadherins Dsg1, Dsg2, Dsg3, Dsc3a, and Dsc3b, and possibly also to Dsc1a and Dsc2a. Two isoforms of Plakophiln-3 have been described that are produced by alternative splicing. Isoform PKP3a is found in desmosomes of most simple and stratified epithelia and PKP3b is abundant in the stratified epithelial cells, but not in simple epithelial cells. Plakophilin-3 levels are up-regulated in lung carcinogenesis. (Ref.: Bonne, S., et al. (1999). J. Cell Sci. 112(1); 2265-2276; Furukawa, C., et al. (2005). Cancer Res. 65(16); 7102-7110).

Immunogen

MBP-tagged recombinant fragment corresponding to the first 308 amino acids from human Plakophilin-3.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin-3 in HaCaT cell lysate.

Specificity

Clone 7F6 specifically detects human Plakophilin-3. It targets an epitope with in the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~95 kDa observed; 87.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).