Anti-ATG5Monoclonal Antibody clone177.19

Analysis Note

ControlWestern Blot and Immunoprecipitation: HeLa cell lysate.

Application

Research CategoryApoptosis & Cancer

Immunoprecipitation: HeLa cell lysate

This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity

Immunogen

Linear Peptide, aa: 8-318

Linkage

Replaces: MABC137

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Quality

Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.

Specificity

Other species have not been tested.

The antibody reacts with the 50 kDa ATG5 protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

50 kDa