Anti-Anosmin

Analysis Note

ControlMCF7cell lysate

Application

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Anosmin in human cerebellum, rat cerebellum, and human thalamus tissues.

Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected Anosmin in MCF7 cells.

Anti-Anosmin Antibody is a highly specific rabbit polyclonal antibody, that targets Anosmin & has been tested in western blotting & ICC.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anosmin is a large (100 kDa) secreted peripheral membrane heparin binding glycoprotein that plays a role in neurodevelopment. Anosmin influences the patterning of the mitral and tufted cell collaterals and stimulates axon elongation and branching in the olfactory system, and it seems to have a critical role in the spatiotemporal arrangement in cranial neural cell formation. The protein interacts with FGFR1 and promotes FGF receptor mediated gene expression but it also inhibits BMP5 and WNT3A signaling activities. It is thought through this orchestration that Anosmin controls the epithelial–mesenchymal transition and the mesenchymal–epithelial transition during neural crest cell development. Mutations in the Anosmin gene, KAL1, are the cause of hypogonadotropic hypogonadism 1. A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. EMD-Millipore's anti-Anosmin rabbit polyclonal antibody has been tested in western blot against human MCF7 cells and immunocytochemistry against MCF7 cells in culture.

Immunogen

KLH-conjugated linear peptide corresponding to human Anosmin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1µg/mL of this antibody detected Anosmin in 15 µg of MCF7 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed. Uncharacterized bands may appear in some lysate(s).