Analysis Note

ControlPOSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.

Application

Research Sub CategoryDevelopmental NeuroscienceNeuroregenerative Medicine

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: Complete sequence

Recombinant full length rat NAP-22.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.

Format: Purified

Specificity

Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.