Analysis Note

ControlPOSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.

Application

Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.

Immunogen

Full length recombinant rat MARCKS.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB5427

Physical form

Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.

Format: Purified

Specificity

Recognizes MARCKS.