MARCKS (COMPLETE); RBX-50UL

Code: AB9298 D2-231

Analysis Note

ControlPOSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates ...


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€529.00 EACH
€650.67 inc. VAT

Analysis Note

ControlPOSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.

Application

Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.

Immunogen

Full length recombinant rat MARCKS.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB5427

Physical form

Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.

Format: Purified

Specificity

Recognizes MARCKS.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... MARCKS(4082)
manufacturer/tradenameChemicon®
NCBI accession no.NM_002356.5
Quality Level100
shipped indry ice
species reactivityrat
technique(s)western blot: suitable
UniProt accession no.P29966
This product has met the following criteria to qualify for the following awards:



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