Analysis Note
ControlPOSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
Application
Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
Immunogen
Full length recombinant rat MARCKS.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Linkage
Replaces: AB5427
Physical form
Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.
Format: Purified
Specificity
Recognizes MARCKS.
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