Analysis Note

ControlProteins tagged with beta galactosidase

Application

Research Sub CategoryEpitope Tags

Anti-β Galactosidase Antibody is an antibody against β Galactosidase for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blotting.

Research CategoryEpitope Tags & General Use

ELISA: 1:10,000-1:50,000 when assayed against 1 µg of Beta Galactosidase in a standard sandwich ELISA.

Immunoblotting: 1:2000-1:10,000 on beta-galactosidase fusion proteins.

Immunohistochemistry: 1:1,000-1:5000 on tissue fixed with 4% paraformaldehyde.

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Beta-Galactosidase (E. coli).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Immunoglobulin Purified by delipidation, salt fractionation and ion-exchange chromatography. Liquid in 0.02M Potassium Phosphate buffer, 0.15M Sodium Chloride, with 0.01% sodium azide.

Format: Purified

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Specificity

Recognizes Beta-Galactosidase (E. coli). Monospecific by IEP. Some cross reactivity with Beta Galactosidase from other species may occur.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.