Application

Detect Chlamydia trachomatis-EB using this Anti-Chlamydia trachomatis-EB Antibody, FITC-conjugated validated for use in IF.

Applications include direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.

Titer by indirect immunofluorescence: >1:1,000 vs. EB′s (all serovars, A-K, L1-L3).

Working dilutions must be determined by end user (suggested starting range 1:10-1:50).

Immunogen

L2 and other serovar groups

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

IgG fraction conjugated with FITC. Supplied in PBS (0.1 M, pH 7.2) with 10 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Specificity

Reacts with purified elementary bodies, disrupted. The unconjugated antibody reacts at >1:1,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3). Cross-reacts with Chlamydia psittacii and Chlamydia pneumoniae (TWAR). Non reactive with HEp-2 cells and egg yolk sac.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months or at 2-8°C for up to 3 months after date of receipt. Avoid repeated freeze/thaw cycles. Store under subdued light.