Application

Research CategorySignaling

Research Sub CategoryGeneral Post-translation Modification

This Anti-Phosphotyrosine Antibody, recombinant clone 4G10™, FITC conjugate is validated for use in IC for the detection of Phosphotyrosine.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to fluorescein isothiocyanate (FITC). Patent pending.

Recognizes: tyrosine-phosphorylated proteins from all species

Molecular Weight: dependent upon the molecular weight of the tyrosine phosphorylated protein being detected

Species Reactivity: wide range

Immunogen

Phosphotyramine-KLH

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

100µg FITC-conjugated recombinant mouse IgG_2bκ in 67µl of PBS containing 0.05% sodium azide. Liquid at 4°C.

Quality

routinely evaluated in immunocytochemistry Immunocytochemistry to detect phosphotyrosine-containing proteins in EGF-stimulated human A431 cells

Storage and Stability

9 months at 4°C