Analysis Note

ControlPositive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Application

Immunocytochemistry:
This antibody is reported to detect Myc-tagged proteins in transfected cells.

Research Sub CategoryEpitope Tags

Research CategoryEpitope Tags & General Use

Anti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Immunogen

Epitope: a.a. 409-420

Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.

Protein A chromatography

Format: Purified

Quality

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Specificity

Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.

Other species cross-reactivity not tested.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.