ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS). However, it is used to greatest advantage with cells cultured in a serum-free and animal component-free environment. This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage. ProFreeze™ requires the addition of 15% reagent or spectrophotometric grade DMSO be added at the time of use. One bottle will make 117.6mL of complete 2X concentrated freezing medium after the addition of 17.6mL DMSO. For best results, keep on ice during use.