Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen, and heparan sulfate proteoglycan. Collagen IV is useful as a substrate for growth of epithelial, endothelial, muscle, and nerve cells. Collagen plays a role in the regulation of cell growth, differentiation and adhesion, as well as tissue formation. Applications include promotion of cell attachment and spreading, cell differentiation and neurite outgrowth, increased proliferation of PC12 cells, studies of effects of collagen IV on cell behavior, and cell adhesion assays.
- Source: Engelbreth-Holm-Swarm (EHS) lathrytic mouse tumor
- Quality control: tested for ability to initiate differentiation (neurite outgrowth) of NG-108 rat glioma/mouse neuroblastoma cells
- Tested and found negative for bacteria and fungi
- Collagen IV purity >90% by SDS-PAGE
- Storage: 2°C to 8°C, do not freeze