I3769-100UG Display Image

Insulin-like Growth Factor-I human; IGF-I; recombinant; expressed in E. coli; lyophilized powder; suitable for cell culture

Code: I3769-100UG D2-231

Analysis Note

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at lea...

read more

€520.00 100UG
List Price

Analysis Note

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg.

Application

Insulin-like Growth Factor-I human has been used:• in modified Dulbecco minimal essential medium for in vitro culture of testicular cells• in culture media, to study its effect on steroidogenesis during testicular development• in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

Biochem/physiol Actions

Insulin-like growth factor I (IGF-I) belongs to the insulin-like growth factor (IGF; see MIM 147440) family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development, especially prenatal growth. IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.

IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.

Physical form

Lyophilized from 0.2 µm filtered solution in 50 mM acetic acid.

Reconstitution

Reconstitute in water

packagingpkg of 50 and 100 µg
Quality Level200
assay≥95% (HPLC)
UniProt accession no.P05019
qualityendotoxin tested
biological sourcehuman
impurities≤1.00 EU/µg
potency0.1-10 ng/mL ED50/EC50
recombinantexpressed in E. coli
formlyophilized powder
application(s)cell culture | mammalian: suitable
mol wt~7.6 kDa
Gene Informationhuman ... IGF1(3479), Igf1(16000)
storage temp.−20°C

Code
Description
Unit Size
List Price
Qty