S2177-.2ML Display Image

Anti-Synaptotagmin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

Code: S2177-.2ML D2-231

Application

Anti-Synaptotagmin antibody produced in rabbit has been used:• for cell surface labelling of synaptotagmin I (SytI) • in immunofluorescence microscopy• in western...

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Application

Anti-Synaptotagmin antibody produced in rabbit has been used:• for cell surface labelling of synaptotagmin I (SytI) • in immunofluorescence microscopy• in western blotting

Biochem/physiol Actions

Synaptotagmin binds Ca2+ phospholipids with high affinity and has a central role in Ca2+ regulated neurotransmitter release. Synaptotagmin functions as a Ca2+ sensor and is required for efficient exocytosis, particularly in the vesicle docking and/or fusion step with the plasma membrane. Ca2+ influx triggers synaptotagmin to interact with either syntaxin or SNAP-25 and the cytoplasmic domain of neurexin leading to fusion and exocytosis. Mutations or deletion of synaptotagmin result in severely impaired Ca2+ triggered neurotransmitter release. Synapses of SytI knockout mice lack the fast-component of Ca2+ dependent neurotransmitter release, but exhibit no changes in the slow, Ca2+ independent component of synaptic vesicle exocytosis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synaptotagmin (Syt, p65) is an abundant synaptic vesicle (SV) membrane protein. It is characterized by a short intravesicular N-terminal domain, a single transmembrane region and two copies of highly conserved internal repeats, known as the C2A and C2B domains, which are homologous to the C2 regulatory region of protein kinase C (PKC) in the cytoplasmic domain. At least eight different isoforms of synaptotagmin (SytI-VIII) are expressed in the brain, four of which (Syt IV, V, VII and VIII) are also expressed in non-neuronal tissues.

Immunogen

synthetic peptide corresponding to N-terminus of synaptotagmin I (SytI) of rat origin (amino acids 1-16 with C-terminally added lysine), conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The sequence is highly conserved among species (SytI) and is not found in other known synaptotagmin isoforms (SytII-VIII).

Quality Level200
clonepolyclonal
antibody product typeprimary antibodies
biological sourcerabbit
application(s)western blot: 1:5,000 using extract of rat brain membrane fraction
application(s)microarray: suitable
conjugateunconjugated
UniProt accession no.P21707
mol wtantigen 65 kDa
Gene Informationhuman ... SYT1(6857)

mouse ... Syt1(20979)

rat ... Syt1(25716)

shipped indry ice
antibody formIgG fraction of antiserum
species reactivityrat
formbuffered aqueous solution
storage temp.−20°C