Anti-interleukin-1β may be used to neutralize the biological activity of recombinant mouse IL-1β on mouse T-helper D10.G4.1 cell line at a concentration of 50 picog/ml. No neutralization of the biological activity of recombinant human IL-1β is observed. The antibody may be used for immunoblotting at a working concentration 1-2 µg/ml or a dilution of 1:1000. In immunoblotting applications the antibody shows 25% cross-reactivity with human recombinant IL-1β.
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Interleukins are proinflammatory cytokines produced on cell injury or trauma. There are two closely related types of interleukins produced by the cell, IL-1a and IL-1ß that are produced by the activation of NF-? B transcription factor in response to bacterial infection or LPS. Both IL-1a and IL-1ß exert their cellular effects by signalling through IL-1 receptor type 1. IL-1ß has pleiotrophic activities and is protective against infections by recruitment of neutrophils to the infection site, secretion of cytokines and chemokines and activation of adhesion molecules. IL-1ß overproduction has been implicated in many auto-immune and allergic disorders such as Cryopirin-associated periodic syndromes, Muckle-Wells syndrome and skin lesions Anti-Interleukin-1ß antibody specifically reacts with mouse IL-1ß. The antibody shows 25% cross reactivity with human recombinant IL-1ß.
recombinant mouse IL-1β.
Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline containing carbohydrates.
Purified using protein G.
The antibody will neutralize the biological activity of recombinant mouse IL-1β, but not recombinant human IL-1β. The antibody shows ~25% cross-reactivity with recombinant human IL-1β.